The A-PON device is a disposable plasma separation device that collects capillary/peripheral whole blood expressed by lancet from a finger, heel, or other appropriate extremity. The device collects the blood passively via a capillary collection tube which is coated with dried EDTA or Li Heparin anticoagulant; the blood mixes with the EDTA to prevent the blood from clotting. As the blood flows passively through the device by capillary action, it passes through a porous membrane that separates blood cells and platelets from the anticoagulated plasma. The plasma collects in the device’s plasma reservoir. Once the plasma reaches the Liquid Color Indicator (LCI) it will change color from white to black which indicates the completion of the collection step. The plasma collection reservoir is connected to an air-filled bulb; when the bulb is squeezed using the user’s fingertip, the plasma is pushed out of the device’s exit aperture into a suitable low-volume and low- analyte binding specimen cup or collection test chamber.